Exosome IV Infusion Therapy

If you aren’t familiar with Exosome IV infusion therapy, this article will keep you updated. We use the therapy to treat joint pain, repair damaged tissues, beauty treatment, and much more. At the end of this article, you will know what Exosome IV infusion therapy is and how it benefits patients. If you still have any queries or need our guidance you can book a consultation with the IV Vitamin Therapy clinic in Los Angeles.

What Are Exosomes?

Exosomes are extracellular vesicles or tiny particles that cells use to share important messages and materials. They range from 30-100 nm diameter in size and are released when a part inside the cell called the multivesicular body (MVB) fuses with the cell’s outer layer. 

Exosomes contain genetic materials like DNA, RNA, and proteins, which are transferred from one cell to another for passing various instructions and influencing the cell’s behavior and functions.

Exosome Formation and Function

During a cell’s endocytosis phase, the cell’s membrane fuses to create endosomes. Inside the endosomes, the exosomes are formed. These exosomes can affect the cells they were released from or the ones they reach. As they travel throughout the body, they relay information and nutrients to the target cells. They perform various activities that influence immune responses, cell proliferation, and neuronal signaling. 

In the field of medicine, they can help stimulate immune responses. They are also considered in the development of vaccines to treat certain cancers [1].

How is the exosome solution sourced?

The traditional methods and kit-based methods are commonly used for exosome isolation. These techniques are crucial for obtaining intact and pure exosome samples, which play essential roles in diagnostics, therapeutics, and research.

Traditional Methods

1. Ultracentrifugation:

This method relies on differential centrifugation to separate exosomes based on their density [2].

• Advantages: Widely used.
• Challenges: Expensive instruments, large sample volumes, protein contamination, and low isolation efficiency.

2. Size-Exclusion Chromatography (SEC):

Separates exosomes based on size using a porous column.

• Advantages: Gentle method.
• Challenges: Limited yield and purity

3. Immunoaffinity:

Antibodies specific to exosomal surface markers capture exosomes.

• Advantages: Specific.
• Challenges: Expensive, low yield, and potential protein contamination^.

4. Ultrafiltration:

Filters exosomes based on size.

• Advantages: Simple.
• Challenges: Sample loss and low efficiency.

5. Precipitation:

Salts or polymers cause exosomes to aggregate and precipitate.

• Advantages: Easy.
  Challenges: Contaminants and low purity.

Kit-Based Methods

1. Commercial Exosome Isolation Kits:

Pre-packaged kits with optimized reagents and protocols.

• Advantages: Convenient, consistent results, and minimal hands-on time.
• Challenges: Cost and dependency on kit quality.

2. Microfluidic Chips:

Miniaturized devices that manipulate fluids at the microscale.

• Advantages: High efficiency, low sample volume, and rapid processing.
• Challenges: Emerging technology, but promising.

Exosome Sources

Exosomes are present in nearly all body fluids, including:

• Blood
• Urine
• Saliva
• Amniotic fluid
• Synovial fluid
• Semen
• Vaginal fluid
• Breast milk
• Serum and plasma from cancer patients

What is Exosome IV infusion?

Exosome IV infusion is a therapy used for joint rejuvenation therapy to treat affected joints. The technique uses concentrated exosomes directly into a patient’s bloodstream. Not only treating joints but exosome infusion can also help accelerate patient’s natural healing processes and speed up recovery from various illnesses and injuries.

Possible Benefits of Exosome IV Infusion Per Ongoing Studies 

1. Tissue Regeneration

Exosome IV therapy has been shown to promote tissue regeneration in health conditions such as tissue damage and musculoskeletal disorders. The exosome, when injected into the affected area, promotes natural healing processes and tissue repair by delivering the nutrients.

2. Protection against various health conditions

Studies have shown the safety, effectiveness, and potential of exosomes in various conditions, including cancers, cardiovascular issues, orthopedic diseases, and many others [3]. Exosome therapy also supports the body’s immune system by promoting the production of new cells and protecting damage against various infections.

3. Enhanced Cognitive Function

A 2015 study conducted on rats suggested that after a traumatic brain injury, using exosome therapy improved their motor function learning ability, reduced inflammation, and generated new cells in the brain [4].

Another 2017 study suggested exosomes derived from mesenchymal stromal cells promote axonal growth of cortical neurons [5].

4. Reduced Inflammation

Exosome IV infusion also has been found to reduce inflammation by targeting the underlying causes and suppressing hypered response from the immune system. 

5. Improved Skin Health

It has been shown to improve collagen production and reduce skin inflammation. When skin collagen is improved, it leads to skin elasticity, which boosts skin firmness and youthfulness. Reduced inflammation can help minimize skin conditions and improve overall skin health.

How long does exosome therapy last?

Regeneration and regrowth are not permanent. Hair loss will continue if you do not treat it to the desired level of growth. Exosomal hair loss treatments last an average of 6 to 9 months. It may take 6-8 months to improve skin collagen and boosts appearance.

Who will administer the exosome infusion, and what qualifications do they have?

• Physicians or Medical Doctors (MDs): MDs have completed medical school and obtained a license to practice medicine. They are trained in various fields, including internal medicine, oncology, cardiology, and regenerative medicine.
  
• Nurse Practitioners (NPs) and Physician Assistants (PAs): NPs hold advanced nursing degrees (Master’s or Doctorate), while PAs complete specialized training programs. Both are licensed to provide medical care under the supervision of MDs.

• Registered Nurses (RNs): RNs have completed nursing education and are licensed to practice. Some may have additional certifications.

• Specialists: Specialists (such as oncologists, neurologists, or cardiologists) have expertise in specific medical areas.

• Laboratory Technicians: Laboratory technicians have training in handling biological samples and operating laboratory equipment.
  
• Certified Medical Assistants (CMAs): CMAs complete certification programs and work under the supervision of medical professionals.

Is exosome injection safe?

Exosomes are naturally occurring vesicles produced by cells. When isolated and purified properly, they are considered safe. Clinical studies have shown minimal adverse effects associated with exosome therapy.

Emerging technologies for efficient exosome isolation

Obtaining intact and pure exosome samples remains a challenge due to the limitations of conventional separation techniques.

Emerging technologies and microfluidic chips offer promising strategies for efficient exosome isolation. These chips can provide affordable and quick separation compared to the traditional methods [6]. 

For future considerations, researchers are keen to explore large-scale GMP production of engineered exosomes. Next-gen Exo-devices hold promising potential for cancer diagnosis and treatment.

Exosome IV Infusion

IV Vitamin Therapy offers the best Exosome IV Infusion near me in Beverly Hills but can also come to your home or office throughout the Los Angeles area. We serve patients near Beverly Hills, Bel Air, West Hollywood, Santa Monica, West Los Angeles, Culver City, Hollywood, Venice, Marina del Rey, Malibu, Manhattan Beach, Redondo Beach, Downtown Los Angeles, Encino, Woodland Hills, Sherman Oaks, Calabasas, Burbank, Glendale, Hidden Hills, Agoura Hills, Northridge, North Hollywood, Topanga, Canoga Park, Reseda, Valley Glen, Chatsworth, West Hills, Winnetka, Universal City, Silverlake, Echo Park, and many more.

References:

1. Rogers, Kara. “exosome”. Encyclopedia Britannica, 8 Sep. 2020, https://www.britannica.com/science/exosome. Accessed 25 January 2024.

2. Wu X, Showiheen SAA, Sun AR, Crawford R, Xiao Y, Mao X, Prasadam I. Exosomes Extraction and Identification. Methods Mol Biol. 2019;2054:81-91. doi: 10.1007/978-1-4939-9769-5_4. PMID: 31482448.

3. Muthu S, Bapat A, Jain R, Jeyaraman N, Jeyaraman M. Exosomal therapy-a new frontier in regenerative medicine. Stem Cell Investig. 2021;8:7. Published 2021 Apr 2. doi:10.21037/sci-2020-037

4. Zhang Y, Chopp M, Meng Y, Katakowski M, Xin H, Mahmood A, Xiong Y. Effect of exosomes derived from multipluripotent mesenchymal stromal cells on functional recovery and neurovascular plasticity in rats after traumatic brain injury. J Neurosurg. 2015 Apr;122(4):856-67. doi: 10.3171/2014.11.JNS14770. Epub 2015 Jan 16. PMID: 25594326; PMCID: PMC4382456.

5 . Zhang, Y., Chopp, M., Liu, X.S. et al. Exosomes Derived from Mesenchymal Stromal Cells Promote Axonal Growth of Cortical Neurons. Mol Neurobiol 54, 2659–2673 (2017). https://doi.org/10.1007/s12035-016-9851-0

6. Chen J, Li P, Zhang T, et al. Review on Strategies and Technologies for Exosome Isolation and Purification. Frontiers in Bioengineering and Biotechnology. 2022;9. doi:https://doi.org/10.3389/fbioe.2021.811971